1ZKY
Human Estrogen Receptor Alpha Ligand-Binding Domain In Complex With OBCP-3M and A Glucocorticoid Receptor Interacting Protein 1 Nr Box II Peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2005-04-07 |
Detector | SBC-3 |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.929, 84.129, 58.220 |
Unit cell angles | 90.00, 109.06, 90.00 |
Refinement procedure
Resolution | 27.010 - 2.250 |
R-factor | 0.18756 |
Rwork | 0.185 |
R-free | 0.23141 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1l2i |
RMSD bond length | 0.011 |
RMSD bond angle | 1.171 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.048 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.118 | 0.564 |
Number of reflections | 24141 | |
<I/σ(I)> | 10.759 | 2 |
Completeness [%] | 59.7 | |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.2M sodium malonate, 20% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |