1ZKP
1.5A Resolution Crystal Structure of a Metallo Beta Lactamase Family Protein, the ELAC Homolgue of Bacillus anthracis, a Putative Ribonuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 99.8 |
| Detector technology | CCD |
| Collection date | 2005-03-18 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.375, 91.190, 78.466 |
| Unit cell angles | 90.00, 115.31, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.502 |
| R-factor | 0.14902 |
| Rwork | 0.147 |
| R-free | 0.18009 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.251 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.570 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.065 | 0.207 |
| Number of reflections | 148486 | |
| <I/σ(I)> | 13.05 | 5 |
| Completeness [%] | 88.0 | 77.7 |
| Redundancy | 8.86 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 298 | 0.2M Ammonoum Sulfate, 0.1M Sodium citrate tribasic dihydrate ph 5.6, 30% w/v PEG 4000 0.5M NaCl, ZnCl, VAPOR DIFFUSION, temperature 298K |
| 1 | VAPOR DIFFUSION | 5.6 | 298 | 0.2M Ammonoum Sulfate, 0.1M Sodium citrate tribasic dihydrate ph 5.6, 30% w/v PEG 4000 0.5M NaCl, ZnCl, VAPOR DIFFUSION, temperature 298K |
| 1 | VAPOR DIFFUSION | 5.6 | 298 | 0.2M Ammonoum Sulfate, 0.1M Sodium citrate tribasic dihydrate ph 5.6, 30% w/v PEG 4000 0.5M NaCl, ZnCl, VAPOR DIFFUSION, temperature 298K |
