1ZKI
Structure of conserved protein PA5202 from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2003-12-09 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97945, 0.97929, 0.96411 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 57.948, 57.948, 113.502 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.000 - 1.700 |
| R-factor | 0.16209 |
| Rwork | 0.160 |
| R-free | 0.19378 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.225 |
| Data reduction software | d*TREK |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.780 |
| High resolution limit [Å] | 1.700 | 1.710 |
| Number of reflections | 23192 | |
| <I/σ(I)> | 2.5 | |
| Completeness [%] | 93.0 | 71.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | magnesium acetate, Tris, PEG3350, glycerol, ethylene glycol, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
