1ZKG

Crystal structure of Transcriptional regulator, TETR family (tm1030) from Thermotoga maritima at 2.30 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-04-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.918370, 0.979508
Spacegroup name	P 1 21 1
Unit cell lengths	50.518, 87.109, 59.156
Unit cell angles	90.00, 110.73, 90.00

Refinement procedure

Resolution	29.180 - 2.300
R-factor	0.20399
Rwork	0.201
R-free	0.26400
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.357
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.180	29.180	2.360
High resolution limit [Å]	2.300	10.290	2.300
Number of reflections	21323
<I/σ(I)>	6	12.2	1.2
Completeness [%]	99.5	96.1	95.8
Redundancy	5	5.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	4.5	293	30.0% PEG-8000, 0.2M MgNO3, 0.1M Citrate pH 4.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K