1ZKD

X-Ray structure of the putative protein Q6N1P6 from Rhodopseudomonas palustris at the resolution 2.1 A , Northeast Structural Genomics Consortium target RpR58

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-04-22
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97913, 0.97942, 0.96774
Spacegroup name	P 61
Unit cell lengths	79.795, 79.795, 247.352
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.190 - 2.100
R-factor	0.223
Rwork	0.223
R-free	0.25800
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	99268
Completeness [%]	99.1	99.1
Redundancy	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	8.5	277	4m Ammonium Acetate, 0.1M Tris-HCl, pH 8.5, microbatch, temperature 277K