1ZKA

NF-kB RelB forms an intertwined homodimer, Y300S mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	105
Detector technology	AREA DETECTOR
Collection date	2002-01-01
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	75.605, 75.605, 65.329
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.230 - 2.200
R-factor	0.206
Rwork	0.206
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdb ID 1zk9
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.050	0.470
Number of reflections	8784
<I/σ(I)>	12.3	2
Completeness [%]	86.0
Redundancy	12	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	PEG 8000,AS, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K