1ZK8
Crystal structure of transcriptional regulator from Bacillus cereus ATCC 14579
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.268, 95.640, 45.986 |
Unit cell angles | 90.00, 97.89, 90.00 |
Refinement procedure
Resolution | 47.840 - 2.150 |
R-factor | 0.18466 |
Rwork | 0.182 |
R-free | 0.23858 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.183 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.250 |
High resolution limit [Å] | 2.150 | 2.150 |
Number of reflections | 18311 | |
Completeness [%] | 94.0 | 92.7 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 15 % Peg 8000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |