1ZK5
Escherichia coli F17fG lectin domain complex with N-acetylglucosamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.811 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.715, 41.959, 53.422 |
| Unit cell angles | 90.00, 97.54, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.400 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.20130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o9v |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.430 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.048 | 0.242 |
| Number of reflections | 32992 | |
| <I/σ(I)> | 15.7 | |
| Completeness [%] | 99.7 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 20% (v/v) isopropanol, 20% PEG 4000, 100 mM Na citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
