1ZJW

Glutaminyl-tRNA synthetase complexed to glutamine and 2'deoxy A76 glutamine tRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-04
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	238.665, 93.465, 114.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.000 - 2.500
R-factor	0.2211
Rwork	0.220
R-free	0.24900
Structure solution method	FOURIER SYNTHESIS
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.580
High resolution limit [Å]	2.300	2.300
Rmerge	0.101
Number of reflections	56902
<I/σ(I)>	6.4
Completeness [%]	99.5
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	Ammonium Sulfate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	Ammonium Sulfate
2	1	1	H2O
3	1	2	Ammonium Sulfate
4	1	2	H2O