1ZJL
Crystal structure of zinc-bound engineered maltose binding protein
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 2005-03-06 |
Detector | UCSD MARK III |
Wavelength(s) | 1.282995 |
Spacegroup name | P 32 |
Unit cell lengths | 80.500, 80.500, 55.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.860 - 2.000 |
R-factor | 0.22 |
Rwork | 0.204 |
R-free | 0.23200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1omp |
RMSD bond length | 0.005 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.065 | 0.148 |
Number of reflections | 27349 | |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 2000 MME, sodium acetate, zinc acetate, HEPES, sodium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |