1ZJK
Crystal structure of the zymogen catalytic region of human MASP-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9392 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.665, 72.689, 110.989 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.900 - 2.180 |
| R-factor | 0.20733 |
| Rwork | 0.205 |
| R-free | 0.25285 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SP domain of PDB ENTRY 1Q3X |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.875 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.900 | 2.250 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.099 | 0.575 |
| Number of reflections | 18330 | |
| <I/σ(I)> | 15.13 | 1.9 |
| Completeness [%] | 88.1 | 43.1 |
| Redundancy | 8.3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG 6000, sodium chloride, TRIS/HCl buffer, pH 8.0, temperature 293K, VAPOR DIFFUSION, HANGING DROP |
