1ZJJ
Crystal structure of hypothetical protein PH1952 from Pyrococcus horikoshii OT3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2004-12-13 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.717, 75.743, 79.784 |
Unit cell angles | 90.00, 103.21, 90.00 |
Refinement procedure
Resolution | 19.970 - 1.850 |
R-factor | 0.195 |
Rwork | 0.195 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wvi |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.057 | 0.217 |
Number of reflections | 40186 | |
<I/σ(I)> | 11.5 | 3.1 |
Completeness [%] | 98.7 | 98.7 |
Redundancy | 3.8 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.8 | 291 | PEG 4000, HEPES, magnesium chloride, pH 6.8, MICROBATCH, temperature 291K |