1ZJI
Aquifex aeolicus KDO8PS R106G mutant in complex with 2PGA and R5P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 84.741, 84.741, 160.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.270 - 2.250 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.24200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1zha |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.110 |
| Rmerge | 0.106 |
| Number of reflections | 35633 |
| <I/σ(I)> | 17.26 |
| Completeness [%] | 91.7 |
| Redundancy | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.78 | 277 | PEG 4K, Na-acetate, pH 4.78, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
