1ZJH

Structure of human muscle pyruvate kinase (PKM2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-04-20
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	I 2 2 2
Unit cell lengths	86.450, 111.290, 126.297
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.110 - 2.200
R-factor	0.231
Rwork	0.231
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pkm
RMSD bond length	0.007
RMSD bond angle	1.100
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.110	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.048	0.568
Number of reflections	31389
Completeness [%]	99.9	100
Redundancy	5.06

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	PEG 3350, Bis-Tris, ammonium acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K