1ZJ8
Structure of Mycobacterium tuberculosis NirA protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.085 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.257, 115.355, 114.697 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.650 - 2.800 |
| R-factor | 0.21866 |
| Rwork | 0.215 |
| R-free | 0.29203 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gep |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.542 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 28218 | |
| <I/σ(I)> | 17.7 | 3.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.3 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG 4000, tris-HCl, magnesium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
