1ZJ6
Crystal structure of human ARL5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6B |
Synchrotron site | Photon Factory |
Beamline | BL-6B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-11-11 |
Detector | RIGAKU |
Wavelength(s) | 1.000 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 76.780, 81.460, 84.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.390 - 2.000 |
Rwork | 0.212 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 500.000 | 2.060 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 16149 | |
Completeness [%] | 88.2 | 91 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 288 | 0.1M sodium citrate (pH5.3), 1.4M ammonium sulfate, 0.1% Triton X-305, VAPOR DIFFUSION, HANGING DROP, temperature 288K |