1ZIO
PHOSPHOTRANSFERASE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-02-05 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.200, 62.165, 41.930 |
| Unit cell angles | 90.00, 117.10, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.960 |
| R-factor | 0.161 |
| Rwork | 0.161 |
| R-free | 0.22800 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 22.390 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | |
| High resolution limit [Å] | 2.000 | |
| Rmerge | 0.106 | |
| Number of reflections | 10949 | |
| Completeness [%] | 90.3 | 90 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7 | 20 * | AMMONIUM SULFATE/PEG 1000, pH 7. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 10-30 (mg/ml) | |
| 2 | 1 | 1 | ammonium sulfate | 2.45 (M) | |
| 3 | 1 | 1 | PEG1000 | 1 (%) | or PEG750 monomethyl ether |
| 4 | 1 | 1 | HEPES | 50 (mM) | |
| 5 | 1 | 1 | sodium azide | 0.1 (%) | |
| 6 | 1 | 1 | Ap5A | 2-5 (mM) |
