1ZI0
A Superhelical Spiral in Escherichia coli DNA Gyrase A C-terminal Domain Imparts Unidirectional Supercoiling Bias
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-01-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9360 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 104.216, 121.432, 177.957 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.300 - 2.600 |
Rwork | 0.227 |
R-free | 0.26600 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.720 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 32428 | |
Completeness [%] | 97.8 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Tris, PEG 1500, NaCl, Sodium Phosphate, EDTA, Beta-Mercaptoethanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Tris, PEG 1500, NaCl, Sodium Phosphate, EDTA, Beta-Mercaptoethanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |