1ZHS
Crystal structure of MVL bound to Man3GlcNAc2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-02-07 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.783, 73.740, 78.863 |
| Unit cell angles | 90.45, 98.45, 108.91 |
Refinement procedure
| Resolution | 19.880 - 1.800 |
| Rwork | 0.192 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.053 | 0.223 |
| Number of reflections | 85237 | |
| <I/σ(I)> | 20.4 | 3.3 |
| Completeness [%] | 91.4 | 68.2 |
| Redundancy | 2.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 295 | Ammonium dihydrogen phosphate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
