1ZHK

Crystal structure of HLA-B*3501 presenting 13-mer EBV antigen LPEPLPQGQLTAY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Collection date	2003-11-25
Wavelength(s)	1.54179
Spacegroup name	P 21 21 21
Unit cell lengths	51.250, 81.750, 110.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.600
R-factor	0.219
Rwork	0.219
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.065	0.485
Number of reflections	60254
<I/σ(I)>	19.6	2.07
Completeness [%]	96.7	84

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	277	0.2M Ammonium Acetate, 17% w/v PEG 3350, 100mM Cacodylate, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K