1ZHH
Crystal Structure of the Apo Form of Vibrio Harveyi LUXP Complexed with the Periplasmic Domain of LUXQ
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1000, 0.9792, 0.9794, 0.9630 |
| Spacegroup name | P 65 |
| Unit cell lengths | 128.468, 128.468, 95.671 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.940 |
| R-factor | 0.207 |
| Rwork | 0.206 |
| R-free | 0.22200 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.012 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Number of reflections | 63780 | |
| Completeness [%] | 96.0 | 92.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 277 | ammonium sulfate, 2-(N-cyclohexylamino)-ethane sulfonic acid, NaCl, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
