1ZHF
Crystal structure of selenomethionine substituted isoflavanone 4'-O-methyltransferase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97932, 0.97942, 0.9855 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 71.355, 71.355, 188.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.700 - 2.500 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.23700 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 2.660 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 19380 | |
| <I/σ(I)> | 7.46 | |
| Completeness [%] | 99.6 | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 277.16 | PEG 8000, amonium acetate, DTT, pH 5.5, temperature 277.16K, VAPOR DIFFUSION |
