1ZHA
A. aeolicus KDO8PS R106G mutant in complex with PEP and R5P
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 84.726, 84.726, 160.433 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.220 - 1.740 |
Rwork | 0.207 |
R-free | 0.23900 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1t96 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 43.220 |
High resolution limit [Å] | 1.740 |
Rmerge | 0.076 |
Number of reflections | 57173 |
<I/σ(I)> | 5.1 |
Completeness [%] | 82.7 |
Redundancy | 14.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | PEG 4K, Na-acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |