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1ZH5

Structural basis for recognition of UUUOH 3'-terminii of nascent RNA pol III transcripts by La autoantigen

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X25
Synchrotron siteNSLS
BeamlineX25
Temperature [K]100
Detector technologyCCD
Collection date2005-02-16
DetectorADSC QUANTUM 315
Wavelength(s)1.1000
Spacegroup nameP 21 21 2
Unit cell lengths156.209, 55.030, 57.782
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution19.920 - 1.850
R-factor0.20252
Rwork0.200
R-free0.24528
Structure solution methodMAD
RMSD bond length0.011
RMSD bond angle1.681
Data scaling softwareHKL-2000
Phasing softwareSHARP
Refinement softwareREFMAC (5.2.0005)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0001.920
High resolution limit [Å]1.8501.850
Rmerge0.496
Number of reflections42919
<I/σ(I)>14.93.7
Completeness [%]99.499.4
Redundancy5.35.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP4.6277PEG 8000, lithium sulfate, potassium chloride, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1VAPOR DIFFUSION, HANGING DROP4.6277PEG 8000, lithium sulfate, potassium chloride, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1VAPOR DIFFUSION, HANGING DROP4.6277PEG 8000, lithium sulfate, potassium chloride, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Crystallization Reagents
IDcrystal IDsolution IDreagent nameconcentrationdetails
111PEG 8000
1012H20
211lithium sulfate
311potassium chloride
411sodium acetate
511H20
612PEG 8000
712lithium sulfate
812potassium chloride
912sodium acetate

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PDB entries from 2024-11-06

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