1ZH5
Structural basis for recognition of UUUOH 3'-terminii of nascent RNA pol III transcripts by La autoantigen
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 156.209, 55.030, 57.782 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.920 - 1.850 |
R-factor | 0.20252 |
Rwork | 0.200 |
R-free | 0.24528 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.681 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.496 | |
Number of reflections | 42919 | |
<I/σ(I)> | 14.9 | 3.7 |
Completeness [%] | 99.4 | 99.4 |
Redundancy | 5.3 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | PEG 8000, lithium sulfate, potassium chloride, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | PEG 8000, lithium sulfate, potassium chloride, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | PEG 8000, lithium sulfate, potassium chloride, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 8000 | ||
10 | 1 | 2 | H20 | ||
2 | 1 | 1 | lithium sulfate | ||
3 | 1 | 1 | potassium chloride | ||
4 | 1 | 1 | sodium acetate | ||
5 | 1 | 1 | H20 | ||
6 | 1 | 2 | PEG 8000 | ||
7 | 1 | 2 | lithium sulfate | ||
8 | 1 | 2 | potassium chloride | ||
9 | 1 | 2 | sodium acetate |