1ZGZ
Crystal Structure Of The Receiver Domain Of TMAO Respiratory System Response Regulator TorR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97888 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.471, 58.389, 64.086 |
| Unit cell angles | 90.00, 112.25, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.19723 |
| Rwork | 0.192 |
| R-free | 0.24370 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.224 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 34815 | |
| <I/σ(I)> | 20.9 | 3.3 |
| Completeness [%] | 97.7 | 80.4 |
| Redundancy | 7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 2000 MME, Ammonium Sulfate, Sodium Acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
