1ZGY
Structural and Biochemical Basis for Selective Repression of the Orphan Nuclear Receptor LRH-1 by SHP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 32-ID |
Synchrotron site | APS |
Beamline | 32-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-05 |
Detector | MARRESEARCH |
Wavelength(s) | 0.99998 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 102.602, 54.493, 66.497 |
Unit cell angles | 90.00, 97.50, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.800 |
R-factor | 0.231 |
Rwork | 0.231 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fm6 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 33433 | |
Completeness [%] | 100.0 | 99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG 3350, tri-sodium citrate, ethylene glycol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |