1ZGX
Crystal structure of ribonuclease mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2003-10-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.096 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.582, 41.513, 58.403 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.900 - 1.130 |
| R-factor | 0.12942 |
| Rwork | 0.128 |
| R-free | 0.16395 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rgg |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.103 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.900 | 1.130 |
| High resolution limit [Å] | 1.120 | 1.120 |
| Rmerge | 0.053 | 0.306 |
| <I/σ(I)> | 31 | 2.7 |
| Completeness [%] | 97.2 | 85 |
| Redundancy | 5.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | ammonium sulfate, Hepes, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
