1ZGW
NMR structure of E. Coli Ada protein in complex with DNA
Spectrometer
Experimental method: SOLUTION NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Varian | INOVA | 900 | |
| 2 | Varian | INOVA | 750 | |
| 3 | Varian | INOVA | 500 | |
| 4 | Bruker | AVANCE | 500 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 7 | Trosy-HNCA |
| 10 | 1 | 5 | 1H-13C HSQC |
| 11 | 1 | 9 | H/D exchange 1H-15N HSQC |
| 12 | 1 | 10 | 15N-separated NOESY (60ms) |
| 13 | 1 | 8 | 13C-separated NOESY (100ms) |
| 14 | 1 | 3 | 13C-separated NOESY (200ms) |
| 15 | 1 | 2 | 2D NOESY |
| 16 | 1 | 6 | 13C-separated NOESY (100ms) |
| 17 | 1 | 4 | 15N-separated NOESY (200ms) |
| 18 | 1 | 9 | 2D NOESY (100ms) |
| 19 | 1 | 10 | 13C-separated NOESY (100ms) in 90% H2O 10% D2O unlabeled protein and 13C Methyl Cys38 |
| 2 | 1 | 7 | Trosy-HN(CO)CA |
| 3 | 1 | 7 | Trosy-HN(CA)CB |
| 4 | 1 | 7 | Trosy-HN(COCA)CB |
| 5 | 1 | 7 | Trosy-HN(CA)CO |
| 6 | 1 | 7 | Trosy-HNCO |
| 7 | 1 | 1 | H(CC_CO)NH-TOCSY |
| 8 | 1 | 1 | (H)C(C-CO)NH-TOCSY |
| 9 | 1 | 8 | HCCH-TOCSY |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 6.5 | ambient | 303 |
Conformers
| Conformers Calculated Total Number | 40 |
| Conformers Submitted Total Number | 20 |
