1ZGT

Structure of hydrogenated rat gamma E crystallin in H2O

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2004-05-11
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9393
Spacegroup name	P 21 21 21
Unit cell lengths	37.200, 43.500, 102.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.300 - 1.450
R-factor	0.205
Rwork	0.200
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a5d
RMSD bond length	0.016
RMSD bond angle	1.689
Data reduction software	MOSFLM
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.300	1.540
High resolution limit [Å]	1.450	1.450
Number of reflections	29137
Completeness [%]	95.6	89.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	PEG 3350, magnesium Actetate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K