1ZGO
High Resolution Crystal Structure of the Discosoma Red Fluorescent Protein (DsRed)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.717, 127.221, 57.118 |
| Unit cell angles | 90.00, 100.42, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.400 |
| R-factor | 0.143 |
| Rwork | 0.143 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.031 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 148353 | |
| <I/σ(I)> | 18 | 1.9 |
| Completeness [%] | 96.8 | 93.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | MPD, HEPES, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
