1ZGN

Crystal Structure of the Glutathione Transferase Pi in Complex with Dinitrosyl-diglutathionyl Iron Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2004-03-21
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9
Spacegroup name	C 1 2 1
Unit cell lengths	76.219, 89.759, 68.713
Unit cell angles	90.00, 97.63, 90.00

Refinement procedure

Resolution	50.000 - 2.100
R-factor	0.182
Rwork	0.182
R-free	0.24400
Structure solution method	Difference Fourier
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	24224
Completeness [%]	90.2	67.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	295.15	MES, PEG 8000, Calcuim chloride, DTT, GSH, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K