1ZGK
1.35 angstrom structure of the Kelch domain of Keap1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Wavelength(s) | 0.979 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 85.745, 85.745, 147.818 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.060 - 1.350 |
R-factor | 0.11175 |
Rwork | 0.111 |
R-free | 0.13264 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.392 |
Data reduction software | HKL-2000 |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.350 |
Rmerge | 0.079 |
Number of reflections | 71295 |
<I/σ(I)> | 86.8 |
Completeness [%] | 99.9 |
Redundancy | 17.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 4% PEG 4000, 100 MM NA HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |