1ZG6
TEM1 beta lactamase mutant S70G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 294 |
| Detector technology | AREA DETECTOR |
| Collection date | 1999-12-13 |
| Detector | UCSD MARK II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.990, 62.650, 89.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.176 |
| Rwork | 0.175 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1btl |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.000 |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.069 |
| Number of reflections | 12889 |
| <I/σ(I)> | 13.7 |
| Completeness [%] | 90.6 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
