1ZFX
The Structure of a minimal all-RNA Hairpin Ribozyme with the mutant G8U at the cleavage site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-30 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 93.350, 93.350, 123.560 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.680 - 2.380 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.25700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1ZFR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data scaling software | CrystalClear |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 2.470 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Number of reflections | 13252 | |
| <I/σ(I)> | 29.9 | 5.2 |
| Completeness [%] | 99.2 | 99.9 |
| Redundancy | 8.7 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 2000 MME, LITHIUM SULFATE, SPERMIDINE, COBALT HEXAAMINE, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 2000 MME | ||
| 2 | 1 | 1 | LITHIUM SULFATE | ||
| 3 | 1 | 1 | SPERMIDINE | ||
| 4 | 1 | 1 | COBALT HEXAAMINE | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | LITHIUM SULFATE | ||
| 7 | 1 | 2 | H2O |
