1ZFV
The structure of an all-RNA minimal Hairpin Ribozyme with Mutation G8A at the cleavage site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-29 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 93.510, 93.510, 124.550 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.770 - 2.400 |
| R-factor | 0.243 |
| Rwork | 0.243 |
| R-free | 0.25300 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1ZFR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.500 |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 13112 | |
| <I/σ(I)> | 27.4 | 4.9 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 11.9 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 2000 MME, LITHIUM SULFATE, SPERMIDINE, COBALT HEXAAMINE , pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 2000 MME | ||
| 2 | 1 | 1 | LITHIUM SULFATE | ||
| 3 | 1 | 1 | SPERMIDINE | ||
| 4 | 1 | 1 | COBALT HEXAAMINE | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | LITHIUM SULFATE | ||
| 7 | 1 | 2 | H2O |
