1ZFP
GROWTH FACTOR RECEPTOR BINDING PROTEIN SH2 DOMAIN COMPLEXED WITH A PHOSPHOTYROSYL PENTAPEPTIDE
Experimental procedure
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR571 |
Temperature [K] | 293 |
Detector technology | DIFFRACTOMETER |
Collection date | 1994-10-21 |
Detector | ENRAF-NONIUS FAST |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 49.900, 113.700, 46.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1LCJ (LCK-SH2) |
RMSD bond length | 0.011 |
RMSD bond angle | 22.481 * |
Data reduction software | MADNES |
Data scaling software | CCP4 |
Phasing software | TFFC |
Refinement software | TNT (4-A) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.093 | 0.440 |
Number of reflections | 12572 | |
<I/σ(I)> | 4.6 | 1.7 |
Completeness [%] | 99.0 | 98 |
Redundancy | 5.6 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10-20 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
3 | 1 | reservoir | PEG400 | 2 (%) | |
4 | 1 | reservoir | HEPES | 0.1 (M) |