1ZFF
TTC Duplex B-DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2003-05-12 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 34.292, 20.318, 33.482 |
| Unit cell angles | 90.00, 112.00, 90.00 |
Refinement procedure
| Resolution | 7.720 - 0.940 |
| R-factor | 0.428 |
| Rwork | 0.428 |
| R-free | 0.44400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NDB ENTRY BD0034 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.280 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 7.900 | 0.970 |
| High resolution limit [Å] | 0.940 | 0.940 |
| Rmerge | 0.046 | 0.218 |
| Number of reflections | 11437 | |
| <I/σ(I)> | 11.2 | 2.3 |
| Completeness [%] | 81.1 | 41.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | Na Cacodylate, CaCl2, Spermine, MPD in resevoir, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K, pH 7.00 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Na Cacodylate | ||
| 2 | 1 | 1 | CaCl2 | ||
| 3 | 1 | 1 | Spermine | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | Na Cacodylate | ||
| 7 | 1 | 2 | CaCl2 | ||
| 8 | 1 | 2 | MPD | ||
| 9 | 1 | 2 | H2O |
