1ZF4
ATC Four-stranded DNA Holliday Junction
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2004-02-26 |
Detector | MARRESEARCH |
Wavelength(s) | 1.072 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 64.936, 24.034, 40.286 |
Unit cell angles | 90.00, 118.69, 90.00 |
Refinement procedure
Resolution | 12.700 - 1.650 |
R-factor | 0.268 |
Rwork | 0.231 |
R-free | 0.27500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | ndb entry UD0058 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.794 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 12.700 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.055 | 0.174 |
Number of reflections | 5414 | |
<I/σ(I)> | 15.5 | 3.8 |
Completeness [%] | 79.3 | 48.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | Na Cacodylate, CaCl2, Spermine, MPD in resevoir, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Na Cacodylate | ||
2 | 1 | 1 | CaCl2 | ||
3 | 1 | 1 | Spermine | ||
4 | 1 | 1 | MPD | ||
5 | 1 | 1 | H2O | ||
6 | 1 | 2 | Na Cacodylate | ||
7 | 1 | 2 | CaCl2 | ||
8 | 1 | 2 | MPD | ||
9 | 1 | 2 | H2O |