1ZF2
Four-stranded DNA Holliday Junction (CCC)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2004-02-26 |
Detector | MARRESEARCH |
Wavelength(s) | 0.827 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 64.960, 24.710, 36.760 |
Unit cell angles | 90.00, 110.69, 90.00 |
Refinement procedure
Resolution | 16.000 - 1.950 |
R-factor | 0.231 |
Rwork | 0.231 |
R-free | 0.27300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NDB ENTRY UD0028 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 16.000 | 2.020 |
High resolution limit [Å] | 1.880 | 1.880 |
Rmerge | 0.103 | 0.335 |
Number of reflections | 3883 | |
<I/σ(I)> | 8 | 2.2 |
Completeness [%] | 88.9 | 65.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | Na Cacodylate, CaCl2, Spermine, MPD in resevoir, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | Na Cacodylate, CaCl2, Spermine, MPD in resevoir, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Na Cacodylate | ||
10 | 2 | 1 | Na Cacodylate | ||
12 | 2 | 1 | CaCl2 | ||
13 | 2 | 1 | Spermine | ||
14 | 2 | 1 | MPD | ||
15 | 2 | 1 | H2O | ||
16 | 2 | 2 | Na Cacodylate | ||
17 | 2 | 2 | CaCl2 | ||
18 | 2 | 2 | MPD | ||
19 | 2 | 2 | H2O | ||
2 | 1 | 1 | CaCl2 | ||
3 | 1 | 1 | Spermine | ||
4 | 1 | 1 | MPD | ||
5 | 1 | 1 | H2O | ||
6 | 1 | 2 | Na Cacodylate | ||
7 | 1 | 2 | CaCl2 | ||
8 | 1 | 2 | MPD | ||
9 | 1 | 2 | H2O |