1ZEW
B-DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2003-06-13 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 63.710, 24.556, 40.631 |
| Unit cell angles | 90.00, 122.59, 90.00 |
Refinement procedure
| Resolution | 17.120 - 2.250 |
| R-factor | 0.244 |
| Rwork | 0.244 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NDB ENTRY BD0028 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.120 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.057 | 0.267 |
| Number of reflections | 3277 | |
| <I/σ(I)> | 17.55 | 1.56 |
| Completeness [%] | 88.6 | 51.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | NA CACODYLATE, CACL2, SPERMINE, MPD, IN RESEVOIR,, pH 7.00, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NA CACODYLATE | ||
| 2 | 1 | 1 | CACL2 | ||
| 3 | 1 | 1 | SPERMINE | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | NA CACODYLATE | ||
| 7 | 1 | 2 | CACL2 | ||
| 8 | 1 | 2 | MPD | ||
| 9 | 1 | 2 | H2O |
