1ZEQ
1.5 A Structure of apo-CusF residues 6-88 from Escherichia coli
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.985 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.300, 39.326, 43.693 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.240 - 1.500 |
| R-factor | 0.18622 |
| Rwork | 0.185 |
| R-free | 0.20674 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.682 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.039 | 0.398 |
| Number of reflections | 11226 | |
| <I/σ(I)> | 74.49 | 5.16 |
| Completeness [%] | 98.9 | 93.1 |
| Redundancy | 10.8 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20% (w/v) PEG, 10% isopropanol, Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
