1ZEN

CLASS II FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Detector technology	IMAGE PLATE
Collection date	1991-09
Detector	MARRESEARCH
Spacegroup name	P 61 2 2
Unit cell lengths	78.360, 78.360, 290.330
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.500
R-factor	0.243
Rwork	0.243
R-free	0.32690 *
RMSD bond length	0.007
RMSD bond angle	23.900 *
Data reduction software	CCP4
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.071
Number of reflections	18922
Completeness [%]	98.0
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *		Kitagawa, Y., (1995) Acta Cryst. D., 51, 833. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	Tris-HCl	100 (mM)
3	1	drop	PEG4000	10-20 (%(w/v))
4	1	drop		0.02-0.3 (M)
5	1	reservoir	PEG4000