1ZEL

Crystal structure of RV2827C protein from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-04
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	87.419, 180.653, 35.112
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.930
R-factor	0.18269
Rwork	0.181
R-free	0.22455
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.376
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.000
High resolution limit [Å]	1.930	1.930
Rmerge	0.076	0.495
Number of reflections	43052
<I/σ(I)>	23.3	3.7
Completeness [%]	99.9	99.8
Redundancy	6.2	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.7	277	0.1 M SODIUM ACETATE, 2 M SODIUM FORMATE, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	4.7	277	0.1 M SODIUM ACETATE, 2 M SODIUM FORMATE, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	4.7	277	0.1 M SODIUM ACETATE, 2 M SODIUM FORMATE, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K