1ZEI
CROSS-LINKED B28 ASP INSULIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.952, 64.772, 48.914 |
| Unit cell angles | 90.00, 109.81, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.1762 * |
| Rwork | 0.176 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | THE MONOCLINIC PHENOL INSULIN DIMER |
| RMSD bond length | 0.019 |
| RMSD bond angle | 0.041 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.054 | 0.216 |
| Total number of observations | 79085 * | |
| Number of reflections | 21942 | |
| Completeness [%] | 87.8 | 77 * |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.4 | 50 * | pH 6.4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | insulin | 3.5 (mg/ml) | |
| 2 | 1 | 1 | 0.02 (M) | 0.5ml | |
| 3 | 1 | 1 | zinc acetate | 0.15 (M) | 0.050ml |
| 4 | 1 | 1 | trisodium citrate | 0.2 (M) | 0.360ml |
| 5 | 1 | 1 | m-cresol in ethanol | 2.5 (%) | 0.200ml |
| 6 | 1 | 1 | 60mg |
