1ZEE
X-Ray Crystal Structure of Protein SO4414 from Shewanella oneidensis. Northeast Structural Genomics Consortium Target SoR52.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97915 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 138.082, 68.024, 90.954 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.500 - 2.310 |
R-factor | 0.234 |
Rwork | 0.234 |
R-free | 0.27500 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.066 | 0.306 |
Number of reflections | 70105 | |
<I/σ(I)> | 17 | 4.46 |
Completeness [%] | 96.5 | 99.6 |
Redundancy | 4.9 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 18% PEG3350, 150 mM lithium sulfate, and 5 mM DTT., pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |