1ZE3

Crystal Structure of the Ternary Complex of FIMD (N-Terminal Domain) with FIMC and the Pilin Domain of FIMH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-15
Detector	MARRESEARCH
Wavelength(s)	0.900
Spacegroup name	P 21 21 21
Unit cell lengths	54.820, 83.320, 110.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.700 - 1.840
Rwork	0.190
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	MODEL DERIVED FROM PDB ENTRY 1QUN
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.615	1.910
High resolution limit [Å]	1.843	1.840
Number of reflections	44185
<I/σ(I)>	15	3.1
Completeness [%]	99.9	99.5
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	PEG 6000, MES buffer, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K