1ZE2
Conformational change of pseudouridine 55 synthase upon its association with RNA substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Wavelength(s) | 0.9793 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 134.975, 134.975, 139.537 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.000 |
Rwork | 0.261 |
R-free | 0.31800 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.000 |
Number of reflections | 26335 |
<I/σ(I)> | 15.7 |
Completeness [%] | 99.4 |
Redundancy | 10.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 278 | PEG6000, pH 8.0, VAPOR DIFFUSION, temperature 278K |
1 | VAPOR DIFFUSION | 8 | 278 | PEG6000, pH 8.0, VAPOR DIFFUSION, temperature 278K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG6000 | ||
2 | 1 | 1 | H2O | ||
3 | 1 | 2 | PEG6000 | ||
4 | 1 | 2 | H2O |