1ZE2
Conformational change of pseudouridine 55 synthase upon its association with RNA substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 134.975, 134.975, 139.537 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| Rwork | 0.261 |
| R-free | 0.31800 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 26335 |
| <I/σ(I)> | 15.7 |
| Completeness [%] | 99.4 |
| Redundancy | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 278 | PEG6000, pH 8.0, VAPOR DIFFUSION, temperature 278K |
| 1 | VAPOR DIFFUSION | 8 | 278 | PEG6000, pH 8.0, VAPOR DIFFUSION, temperature 278K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG6000 | ||
| 2 | 1 | 1 | H2O | ||
| 3 | 1 | 2 | PEG6000 | ||
| 4 | 1 | 2 | H2O |
