1ZD1
Human Sulfortransferase SULT4A1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-03-23 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.938, 74.195, 85.832 |
| Unit cell angles | 90.00, 105.52, 90.00 |
Refinement procedure
| Resolution | 82.760 - 2.240 |
| R-factor | 0.18977 |
| Rwork | 0.186 |
| R-free | 0.26239 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.050 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.000 | 2.280 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.870 | 0.600 |
| Number of reflections | 28728 | |
| <I/σ(I)> | 9.2 | 2.55 |
| Completeness [%] | 99.1 | 97.6 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 300 | 20% PEG3350, 0.2M Ammonium Tartrate, VAPOR DIFFUSION, temperature 300K, pH 6.5 |
