1ZCK
native structure prl-1 (ptp4a1)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 200 |
Detector technology | IMAGE PLATE |
Collection date | 2003-06-30 |
Detector | RIGAKU RAXIS II |
Wavelength(s) | 0.9800, 0.97849, 0.96261 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 71.291, 105.568, 181.355 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.900 |
Rwork | 0.245 |
R-free | 0.25700 |
Structure solution method | MAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 48966 | |
Completeness [%] | 96.9 | 88.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 277 | Ammonium Sulfate, Sodium Acetate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |