1ZC6
Crystal Structure of Putative N-acetylglucosamine Kinase from Chromobacterium violaceum. Northeast Structural Genomics Target Cvr23.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9876 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 178.245, 178.245, 117.663 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.420 - 2.200 |
R-factor | 0.241 |
Rwork | 0.241 |
R-free | 0.26700 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.104 | 0.863 |
Number of reflections | 90884 | |
Completeness [%] | 95.4 | |
Redundancy | 10.1 | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.15-0.25 M magnesium chloride, 15-20% PEG 3350, 0.1 M MES , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |